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试剂耗材

Advanced DMEM/F-12, 12634010

产品介绍【返回】

品牌: gibco
货号: 12634010
供应商: 上海觅拓
保质期: 12个月
保存条件: 2-8度,避光
规格: 500ml

描述

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.
This Advanced DMEM/F-12 is manufactured as follows:
With Without
• Glucose • L-glutamine
• Non Essential Amino Acids • HEPES
• Sodium pyruvate  
• Phenol Red  

The complete  is available.
Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5%  and 4 mM L-glutamine or supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
For supply chain continuity, we manufacture Advanced DMEM/F-12 at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
 

规格

Glutamine: No Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Serum Supplementation: Advanced (Reduced Serum)
Glucose: High Glucose
Product Line: Gibco®
Product Size: 500 mL
Volume (Metric): 500 ml
Green Features: Sustainable packaging
Shipping Condition: Room Temperature

内容及储存

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
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